Figure 7.

shRNA knockdown of PXK disrupts BCR colocalization. (A) PXK protein expression following shRNA knockdown in 5 LCL lines from non-lupus subjects (expression was assessed in triplicate for each cell line). (B) Summary of BCR internalization at 2 min in LCLs following viral transduction with either an shRNA against PXK or a scrambled control. Results from unpaired Student's t-test shown. (C) Correlation between PXK protein expression and BCR internalization following shRNA knockdown. The significance was assessed using a Pearson correlation. The graphs in (B,C) are representative of the results of three independent experiments.