Table 1.
Quantitative Polymerase Chain Reaction Primers
| Target bacteria | Primer sequence 5′-3′ | Annealing temperature (°C) | PCR product size (base pair) |
|---|---|---|---|
| Bacteroides spp. | F- ATAGCCTTTCGAAAGRAAGAT | 50 | 495 |
| R- CCAGTATCAACTGCAATTTTA | |||
| Clostridium spp. | F- CGGTACCTGACTAAGAAGC | 50 | 429 |
| R- AGTTTYATTCTTGCGAACG | |||
| Escherichia coli | F- GTTAATACCTTTGCTCATTGA | 52 | 340 |
| R- ACCAGGGTATCTAATCCTGTT | |||
| Bifidobacterium spp. | F- GGGTGGTAATGCCGGATG | 55 | 442 |
| R- TAAGCGATGGACTTTCACACC | |||
| Lactobacillus spp. | F- AGCAGTAGGGAATCTTCCA | 50 | 341 |
| R- CACCGCTACACATGGAG |