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. 2015 Jan 9;8:438. doi: 10.3389/fncel.2014.00438

Figure 1.

Figure 1

Tunicamycin triggers unfolded protein response in hippocampal slices. (A) Western Blot for BIP/GRP78 after treatment with DMSO (vehicle) or Tunicamycin 10 μg/mL (T10) and 80 μg/mL (T80) for 24 h. Western blot for ERK was used as loading control. (B) Quantification of optical density (O.D). Values represent ± S.E.M. compared to vehicles *p < 0.1; **p < 0.05. ANOVA was performed followed by Bonferroni's post-test. P < 0.01, C.I.: 99.9%, N = 4 independent experiments. (C–E) Immunofluorescence for CHOP/GADD153 (green) and counterstaining of DNA with TO-PRO3 (blue) after Tunicamycin treatment at 10 (D) or 80 μg/mL (E) or vehicles (C). Note a nuclear localization of CHOP/GADD153. Scale bar: 20 μm.