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. 2015 Jan 9;8:438. doi: 10.3389/fncel.2014.00438

Figure 3.

Figure 3

Transgene distribution in hippocampal slices after rAAV8 infection. Hippocampal slices were infected with rAAV8-GFP or rAAV8-CHIP and maintained for 14 days in vitro. (A) Representative photomicrography of slice infected in day in vitro 0 (DIV0) 14 days after rAAV8-GFP infection. 4x Magnification; scale bar: 500 μm. (B) Higher magnification (40x) of selected region in (A). GFP fluorescence (green) and TOPRO3 (blue). (C,D) Immunofluorescence for CHIP (white) counterstained with TO-PRO3 (blue) comparing non-infected (C) with rAAV8-CHIP infected slice (D); 63x magnification, scale bar: 20 μm. (E) Western blot for CHIP of Tunicamycin treated slices at 10 (T10) or 80 μg/mL (T80) compared to vehicles (DMSO). (F) Western blot comparing samples infected with rAAV8-CHIP to non-infected slices. (E,F) Western blot for ERK-2 was used as loading control.