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Overexpression of CHIP diminishes p53 positive cells in hippocampal slices treated with Tunicamycin. (A–C) Immunofluorescence for p53 (in red) counterstained with nuclear marker TO-PRO3 (in white) shows increased expression in treated slices (B–B″) compared to vehicles (A–A″) and rAAV8-CHIP+ Tunicamycin treated slices (C–C″). (D) Quantitative analysis of p53+ cells/area ***p < 0.0001. Values represent ± S.E.M. compared to vehicles. P < 0.01, C.I.: 99%, N = 3 independent experiments.
