Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 3;43(1):115–128. doi: 10.1093/nar/gku1185

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — MCM5 middle meiosis-specific isoform expression. (A and B) Heatmaps and RNA-Seq data for MCM5 are given as in Figure 3. (C) A schematic shows the Northern blot target locus and primer positions within the ORF. Mitotic (MCM5) and meiotic (mMCM5) isoforms are given. ACT1 was used as a loading control. (D) 5′-RACE PCR data are shown for MCM5 isoforms in rich media (YPD, YPA) and three samples covering the induction of the meiotic isoform (SPII 2, 6, 8 h). (E) A western blot is shown for myc-tagged Mcm5 (Mcm5^myc) from samples as in panel C except that the 12-h time point in SPII was replaced by a 24-h time point. Pgk1 was used as a loading control. A histogram is shown as in Figure 3D.