Figure 2.

Budding defects of ΔZF2 HIV are not the consequence of late RTion activation. Budding events were studied by TIRFM and analyzed as performed in Figure 1, in the absence of late RT activity. Biogenesis of WT and ΔZF2 viruses in cells was indeed measured in the presence of AZT. For VLP formed by Gag alone (without Pol proteins), cells were transfected 6 h with Gag plasmids. ***P < 0.001, as determined by the Mann–Whitney U-test.