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. 2014 Dec 8;43(1):336–347. doi: 10.1093/nar/gku1232

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Tsg101 strengthens the control of late RTion in the producer cells. (A) Effect of Tsg101 on nucleic acid content of virions. Virus-containing supernatant produced in the trans-complementation experiments were collected and the viral nucleic acid content was monitored by RT-qPCR and qPCR. Results are presented as the number of DNA (top) or RNA (bottom) copies measured in the same sample of total virus produced. The virus populations from at least three independent transfections were analyzed and values plotted using a at log₁₀ scale and displayed as mean ± SEM. ***P < 0.001. (B) Quantitation of the late RTion activity in producer cells. Amount of viral MS cDNA produced by late RTion in producer cells was determined by qPCR as in (A). The number of DNA copies was normalized to cellular GAPDH gene and displayed as mean ± SEM.