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. 2014 Dec 8;43(1):336–347. doi: 10.1093/nar/gku1232

Figure 7.

Figure 7.

Tsg101 depletion activates late RTion in WT HIV producing cells. (A) Downregulation of Tsg101 expression by siRNA. 293T cells were cotransfected for 48 h with pNL4-3Δenv and 10 or 50 nM siRNA targeting or not Tsg101. Cell lysates were analyzed by anti-Tsg101 and anti-p24 western blots. (B) siRNA-mediated Tsg101 downregulation activated late RTion in cells producing WT HIV. Cells were similarly transfected and late RTion activity was determined as described in Figure 6 and compared to late RTion activity measured in WT HIV producer cells without siRNA. ***P < 0.001.