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. 2014 Nov 30;43(1):237–246. doi: 10.1093/nar/gku1257

Figure 2.

Figure 2.

Cascade-mediated control of transcription. (A) Schematic drawing showing the positions of protospacers in the PLtetO-1 promoter and the gfp ORF (not drawn to scale). (B) E. coli cells expressing Cascade with P3 crRNA display Gfp silencing (left), compared to cells expressing Cascade with non-targeting crRNA (right). (C) Optical density analysis of cultures grown in 96-well plates demonstrates little difference in growth between strains expressing Cascade targeted to different regions of the PLtetO-1 promoter and strain expressing non-targeting Cascade (indicated by ‘C’). (D) Gfp fluorescence of cultures grown in 96-well plates expressing Cascade guided to different regions of the PLtetO-1 promoter is dramatically lower than of cultures expressing non-targeting Cascade, demonstrating efficient silencing of gfp. (E) Relative Gfp fluorescence of cultures expressing Cascade guided to different parts of the promoter 3 h after induction compared to cultures expressing Cascade and non-targeting crRNA. (F) Relative Gfp fluorescence of cultures expressing Cascade guided to different part of the gfp ORF 3 h after induction compared to cultures expressing Cascade and non-targeting crRNA. Target is plasmid encoded in all experiments. Error bars represent SD.