Figure 6.

Cascade binding interferes with transcription. Northern blot analysis of full-length gfp transcript abundance during Cascade-crRNA expression. The crRNAs used are indicated above the blot; +/- indicate presence/absence of gfp, Cascade or crRNA. 5S rRNA was used as loading control. (A) No gfp transcripts are detected when promoter-targeting crRNA and Cascade are expressed. (B) Cells expressing Cascade and NT1, T1 or T2 crRNA have decreased gfp transcript levels compared to cells expressing Cascade and non-targeting crRNA (indicated by ‘C’) while NT2 provides no reduction, in accordance with fluorescence data (Figure 2).