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Schematic diagrams for PARE, C-PARE and SPARE library construction. (A) PARE, (B) C-PARE and (C) SPARE libraries were constructed from poly(A)+ RNA in multiple steps (red, green or blue arrows). The procedures used to construct all of the libraries are shown in bold lettering, while those used to construct specific libraries are shown in regular lettering. 5′ m7Gppp, capped 5′ end; 5′ P monophosphorylated 5′ end; 5′ OH, hydroxylated 5′ end; (A)n, polyadenylated 3′ end.
