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. 2014 Nov 27;43(1):309–323. doi: 10.1093/nar/gku1258

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Representative D-plots of endogenous SMG6 targets identified by PARE. A transcript map is shown above each set of D-plots. Alternating gray and white zones represent exons. AUG, translation initiation codon; TC, natural termination codon; uAUG, predicted translation initiation codon of an upstream ORF; uTC, predicted termination codon of an upstream ORF; SelCys, selenocysteine codon. (A) RAE1–002, a transcript with a long 3′ UTR. (B) IFRD1–001, a transcript with a predicted uORF. (C) GPX1–001, a transcript with a selenocysteine codon. (D) ENY2–009, a transcript with an exon junction downstream of the TC. All of the D-plots were drawn with data from Biorep 1; similar effects were observed for Biorep 2.