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. 2014 Dec 10;43(1):40–50. doi: 10.1093/nar/gku1268

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Time-resolved fluorescence decay of Bimane. (a) Decay curve in the trimolecular complex L(dAp)ER(Bim) at 45 mM [Mg²⁺]. The decay curve was fit with three lifetime components; (b–d) [Mg²⁺] dependence of the single fluorescence lifetime components and of the amplitude averaged fluorescence lifetime (black squares, , red circles, , blue triangles, and black circles, ); (e) fractional amplitudes α; and (f) fractional intensities () of the three decay components; Conditions: 4.2 μM L(dAp), 4.1 μM E, 4.0 μM R(Bim), pH 7.5; 15°C; λ_exc = 405 nm, λ_em = 480 nm.

Inline graphic — Time-resolved fluorescence decay of Bimane. (a) Decay curve in the trimolecular complex L(dAp)ER(Bim) at 45 mM [Mg²⁺]. The decay curve was fit with three lifetime components; (b–d) [Mg²⁺] dependence of the single fluorescence lifetime components and of the amplitude averaged fluorescence lifetime (black squares, , red circles, , blue triangles, and black circles, ); (e) fractional amplitudes α; and (f) fractional intensities () of the three decay components; Conditions: 4.2 μM L(dAp), 4.1 μM E, 4.0 μM R(Bim), pH 7.5; 15°C; λ_exc = 405 nm, λ_em = 480 nm.