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. 2014 Dec 10;43(1):40–50. doi: 10.1093/nar/gku1268

Figure 7.

Figure 7.

Time dependent anisotropy of Bim: dependence on the concentration of Mg2+. (a) Anisotropy decay of Bim in the L(dAp)ER(Bim) trimolecular complex at 45 mM MgCl2, decay curve analyzed with Equation (5); (b) residual anisotropy Inline graphic; (c) rotational correlation time ϕ. Conditions: 4.2 μM L(dAp), 4.1 μM E, 4.0 μM R(Bim), pH 7.5; 15°C; λexc = 405 nm, λem = 480 nm.