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. 2014 Dec 17;43(1):618–628. doi: 10.1093/nar/gku1270

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Cryo-ET reconstruction of two topologically different hexasomes formed on capped polyadenylated N-tagged GFP-encoding mRNA, 975-nt coding sequence (construct 1, see Materials and Methods) after 15 min translation. (A) The hexasome with circular mRNA path, visible as short double-row polyribosome. (B) The hexasome with topologically linear, zigzag-like mRNA path, also visible as short polyribosome with two parallel rows of ribosomes (‘pseudo-double-row polysome’).