Figure 3.

Decreases in candidate RNA levels occur early in mRNA biogenesis and are not due to decreased stability. (A) Schematic of uridine analog RNA labeling experiments; see text for details. (B) Analysis of newly made transcript levels by RT-qPCR in a 4SU labeling experiment. The ‘-4SU’ samples were transfected with siControl but no 4SU was added during the pulse. (C) Decay curves of candidate transcripts following transcription inhibition with 1 μg/ml ActD. Dashed lines represent cells treated with siREF and solid lines represent cells treated with siControl. Experiments in (B) and (C) were performed three times and error bars represent standard deviation from the mean.