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. 2014 Dec 10;43(1):247–258. doi: 10.1093/nar/gku1279

Figure 6.

Figure 6.

CSB promotes DNA ICL processing. (A) Unhooking of DNA ICLs in non-cycling scramble shRNA and CSB shRNA SH-SY5Y cells. Designated cells received no treatment or were treated with trioxsalen/UVA and fixed immediately (0 h) or 8 h later. The box plots (above) show the tail moments determined from analysis of more than 150 nuclear DNAs from at least three different experiments. Error bars indicate SD, and P-values were determined by statistical analysis using the Mann–Whitney U-test. Representative Comet images are shown (below) for the indicated treatments, cells and time points. (B) γ-H2AX foci formation and disappearance following trioxsalen/UVA treatment of non-cycling scramble shRNA or CSB shRNA SH-SY5Y cells. (Top) Representative images of γ-H2AX staining (red) and DAPI (blue) at the indicated time after trioxsalen/UVA treatment are shown. (Bottom) The percentage of γ-H2AX positive cells (>10 foci per cell) was quantified from more than 200 cells of two different preparations. Statistical significance between the values obtained for scramble shRNA and CSB shRNA SH-SY5Y cells was determined using Fisher's exact test and Bonferroni correction. *P < 0.05, **P < 0.01, ***P < 0.001.