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. 2014 Dec 4;43(1):520–529. doi: 10.1093/nar/gku1281

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Involvement of premature transcription termination in the FMN riboswitch function. (A) Schematic of targets of ChIP-qPCR analysis. A chromosomal region around the ribM gene is represented. Regions coding the FMN riboswitch and ribM CDS are indicated as a box and an arrow, respectively. Numbers indicate the distance from the transcription start point of ribM, which is designated as ‘1’. Bars above the diagram are targets of ChIP-qPCR (RFN1; 26–107 nt, RFN2; 69–131 nt, CDS; 492–548 nt, CDS2; 692–790 nt, Cterm; 832–911 nt) (B–D) ChIP-qPCR analysis. Relative RNAP occupancies (Rel. RNAP Occ.) at each target region (horizontal axis) in −FMN (white bars) and +FMN (black bars) cells of the ΔribA rpoC-FLAG (TK13) (B), ΔribA CAmut rpoC-FLAG (TK14) (C) and Δrho ΔribA rpoC-FLAG (TK15) (D) strains. The values shown are means from at least three experiments and standard deviations. (E) Relative expression levels of ribM mRNA (left) and RibM protein (right) in −FMN cells (white bars) and +FMN cells (black bars) of the ΔribA ribM-FLAG (TK05) and Δrho ΔribA ribM-FLAG (TK17) strains. The strains are indicated along the horizontal axis (ΔribA, TK05; and Δrho ΔribA, TK17). The values shown are means from at least three experiments standard deviations.