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. 2014 Dec 4;43(1):544–552. doi: 10.1093/nar/gku1289

Figure 1.

Figure 1.

Activation of OAS1 by adenovirus VA RNAI requires its single-stranded pyrimidine-rich 3′-end. (A) VA RNAI secondary structure and domain organization. The Terminal Stem 3′-end contains a single-stranded sequence (CUUU-3′; dashed box and shown as sequence in subsequent panels). (B) Chromogenic assay of OAS1 activity shows that deletion of the wild-type 3′-end single-stranded sequence (Δ3′; dashed line) dramatically reduces OAS activation by full-length VA RNAI (CUUU-3′; solid line). (C) Alternate pyrimidine-rich (UCCC-3′) or purine-rich (AGGG-3′ or GAAA-3′) single-stranded 3′-ends promote full and partial activation of OAS1, respectively. Addition of a complementary 5′-end extension to fully base pair the UCCC-3′ sequence (5′-AGGG/UCCC-3′) attenuates OAS1 activation to the same extent as when the 3′-end single-stranded sequence is absent. The data in panels (B) and (C) are normalized to wild-type VA RNAI (CUUU-3′).