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. 2014 Dec 9;43(1):162–178. doi: 10.1093/nar/gku1293

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — TGFβ-induced EMT is associated with epigenetic changes on the Cdh1 promoter. (A) Morphology of parental NMuMG cells untreated, treated with TGFβ1 (1 ng/ml) for the indicated time periods, or treated with TGFβ1 for 22 days with subsequent withdrawal of TGFβ1 for another 14 days (WD). Scale bar: 50 μm. (B) Immunoblot analyses for E-cadherin and fibronectin protein levels in cells as described in (A). GAPDH and α-tubulin serve as loading control. (C) Binding of endogenous HMGA2, Snail and DNMT3A on the mouse Cdh1 promoter analysed by ChIP-qPCR in cells described in (A). (D) ChIP-qPCR analyses of active H3K4me3 marks and repressive H3K9me3 and H3K27me3 marks at the Cdh1 promoter in cells described in (A).