Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 8;43(1):259–271. doi: 10.1093/nar/gku1294

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 3. — LKB1 regulates BRCA1 mRNA stability. (A) U2OS-DRGFP cell lines stably expressing a non-specific (NS) shRNA or LKB1 shRNAs were analyzed for the expression of LKB1 transcript and protein by RT-qPCR (top) and immunoblot (bottom), respectively. (B) U2OS-DRGFP cell expressing indicated shRNAs was analyzed for HR frequency by monitoring for GFP expression after 48 h of I-Sce1 transfection. Relative HR frequencies under indicated conditions are plotted. (C) The mRNA level of BRCA1 was measured by RT-qPCR in cells expressing LKB1 shRNAs or a non-specific (NS) shRNA that were either unirradiated or gamma-irradiated (20G). Actin expression was analyzed as the internal control. (D) The protein level of BRCA1 was measured by immunoblot analysis in cells expressing LKB1 shRNAs or the NS shRNA that were either unirradiated or gamma-irradiated (20G). Actin was measured as loading control. (E) The half-life of BRCA1 mRNA was measured at indicated time points after irradiation (20 Gray) and treatment with the transcription blocker, actinomycin D (5 μM), in cell lines that either expressed LKB1 shRNA or the non-specific shRNA. The relative BRCA1 mRNA abundance was normalized to actin at the indicated time points and is plotted. The BRCA1 half-lives were significantly different in cells expression NS shRNAs compared to the cells expressing LKB1 shRNAs (P < 0.01). Error bars show standard error mean (SEM). (*P < 0.01; **P < 0.001).