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. 2014 Dec 10;43(1):282–294. doi: 10.1093/nar/gku1301

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Colony survival and replication fork analysis of Polh^−/−; Polk^−/− MEFs. (A) Colony survival after MMS treatment of SV40 immortalized WT, Polh^−/−, Polk^−/− and Polh^−/−; Polk^−/- cells. Colony survival was normalized to the mock-treated cells for each condition and corrected for plating efficiency. Average of at least four independent experiments is plotted ±SD. The dashed line indicates 50% survival. Values behind the genotype description indicate average plating efficiencies ±SD. (B) Replication speed on non-damaged CldU track of SV40 immortalized WT, Polh^−/−, Polk^−/− and Polh^−/−; Polk^−/- cells. Average of at least 50 analyzed DNA fibers is shown ±SD. (C) Replication fork stalling analysis as in Figure 3C of SV40 immortalized WT, Polh^−/−, Polk^−/− and Polh^−/−; Polk^−/- cells. Data are presented as cumulative percentage of forks at each ratio. At least 50 DNA fibers were analyzed per experiment. The Kolmogorov–Smirnov (K-S) test was performed to determine statistical significance. NS means not significant. Only MMS-treated Polh^−/−; Polk^−/− cells show a significant difference (K-S test, p < 10⁻⁴) compared to MMS-treated wild-type cells. A representative experiment (of 2) is shown.