Figure 2.

Identification of a trinucleotide sequence motif in crRNA important for RNA interference. Complete sequence of S1 is shown on the top of the left panel and numbered. S1 spacer mutants are annotated according to the positions of their mutations in the spacer and the mutated bases are shown in the left panel. The right panel illustrates RNA targeting activity of the wild-type S1 spacer (100%) and mutated derivatives relative to the wild-type spacer. Spacer mutants showing a greatly reduced RNA targeting activity are highlighted in colours. Three independent transformants were analysed for each construct with bars indicating standard deviations.