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. 2014 Dec 12;43(1):1–12. doi: 10.1093/nar/gku1303

Figure 1.

Figure 1.

Preparation of Southern type microarrays and hybridization to tRNAPhe. (A) schematic pattern of preparation of microarray and distribution of oligonucleotide probes on it, (B) hybridization of target RNA in 3.5 M TMACl at 4°C to microarray in reverse synthons manner, (C) hybridization of target RNA in 1 M NaCl/10 mM MgCl2 at 4°C to microarray in a reverse synthons manner and (D) hybridization of target RNA in 3.5 M TMACl at 4°C to microarray in a standard synthons manner. Reprinted with permission from (21). Copyright 1999 Macmillan Publishers Ltd.