Figure 2.

Organization of regulatory elements in target promoters negatively regulated by AdcR. The positions of predicted -10 and -35 hexamers, and the TSS (bent arrow) in the AdcR-regulated promoters containing one (A) and two (B) AdcR-binding sites are shown. The locations of adc motifs are indicated in red. Interactions between the metallated form of AdcR and operator sequences containing one (C) and two (D) adc motifs as assessed by gel mobility shift assay. Increasing concentrations of AdcR (250, 500 and 750 ng) were incubated with the respective oligoduplexes and reactions mixtures were resolved on a 10% native PAGE. The positions of free (F) and AdcR-bound (B) forms of probe are indicated.