Figure 7.

Metal binding ligands of AdcR are critical for AdcR-DNA interactions. (A) DNA binding activites of WT and mutant derivatives of AdcR, as assessed by gel mobility shift assay. Increasing concentrations of purified WT or AdcR mutant proteins were incubated with oligoduplexes containing the adc motif and resolved on native PAGE. The positions of unbound (F) and AdcR-bound (B) probes are indicated. Coomassie-stained bands corresponding to purified WT and mutant derivatives of AdcR are shown. Taqman qRT-PCR analysis of adcC (B), phtD (C) and hasA (D) transcript levels in indicated strains relative to constitutively expressed endogenous control, tufA. Data graphed are mean ± standard deviation for three biological replicates.