Figure 2.

CBR-CSR-1 pathway members are highly expressed during reproductive stages and are required for embryonic viability. (A) Developmentally staged wild-type AF16 C. briggsae samples were subjected to western blotting for CBR-CSR-1 protein. Tubulin is shown as a loading control. L1, L2, L3 and L4 are larval stages, YA = young adult hermaphrodites lacking embryos, GA = mature adult hermaphrodites possessing embryos, Embryo = mixed stage embryo, GA (C.e.) = C. elegans mature adult hermaphrodite protein as a positive control. (B) Quantitative real-time PCR analysis of the mRNA expression of CSR-1 pathway components throughout wild-type development (strain AF16). Levels are expressed as arbitrary values, relative to actin mRNA, developmental stages are as in (A), error bars represent standard deviation of triplicate experiments.