Skip to main content
. 2014 Dec 15;43(1):595–606. doi: 10.1093/nar/gku1311

Figure 4.

Figure 4.

Promoting G4 folding. (A) A schematic representationof the ASO-based strategy to promote long-loop-2 G4 folding against the larger nucleotidic context. (B) Sequences of the double-stranded G4 (DsG4) and Pro-DsG4 ASO. The G4 nucleotides are capitalized. The guanine residues which were mutated for adenines in the G/A-mutant are shown directly above the respective DsG4 residues. Nucleotide numbering is shown directly above the DsG4 sequence. The Pro-DsG4 oligonucleotide sequence is located directly beneath the complementary DsG4 nucleotide stretch. (C) Histograms showing the intensity ratio K+/Li+, which is an accurate reflection of relative accessibility, for each nucleotide of the DsG4 and G/A-mutant at three different concentrations of Pro-DsG4. The average of two independent experiments is shown. (D) Luciferase activity in HEK293 cells for the DsG4 and G/A-mutant. (E) and (F) Relative levels of G4 formation obtained by comparing the G/A mutant and DsG4 ratios of luciferase activity with (E) a control and the Pro-DsG4 ASO and (F) a control and the H2AFY-G4. The G/A mutant/wild type ratio with the control ASO was set at 100, and a ratio equal to 1 was set at 0. Means and s.d. were calculated from four different experiments, each conducted in triplicate.