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. 2014 Dec 15;43(1):595–606. doi: 10.1093/nar/gku1311

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Targeting endogenously expressed H2AFY. (A) Typical western blot analysis of Caco-2 cells for the H2AFY protein. Histone 3 (H3) was used as a loading control. Cells were transfected either with the Control, Anti-H2G4 or Pro-H2G4 ASO. (B) Quantification of the western blots. Means and SD were calculated from three independent experiments, each conducted in triplicate. (C) Relative endogenous mRNA levels for H2AFY isoform 1 (left panel) and isoforms 1, 3 and 4 taken together (right panel) in Caco-2 cells transfected with either the Control (green), Anti-H2G4 (Light green) or Pro-H2G4 (dark green) ASO. Means and SD were calculated from three independent experiments, each conducted in triplicate.