Figure 4.

LQ and 2-linker cloning methods isolate small RNAs from a synthetic miRNA mixture and total RNA from human blood plasma. (A) The frequency of small RNA read lengths obtained from libraries generated with decreasing amounts of the synthetic miRNA mixture using the LQ method (black text) and the 2-linker method (gray line, red text). Library designation indicated in (). Where multiple libraries are indicated, the distribution is shown as the average of these libraries. (B) The frequency of small RNA read lengths in libraries generated using total RNA isolated from human blood plasma is represented as the average across all plasma libraries with error bars indicating the standard deviation.