Figure 3.

Activation of eosinophils differentiated from hematopoietic precursors in the additional presence of IFN-γ. CD34⁺-enriched hematopoietic progenitor cells were cultured for three days with SCF, TPO, Flt3L, IL-3, and IL-5, after which they were cultured for three additional weeks with just IL-3 and IL-5 with or without the additional presence of IFN-γ. Eosinophils matured from CD34⁺ progenitors were activated with or without calcium ionophore/PMA, LysASA (1mM and 10mM) or ketorolac (1mM and 10mM) for 30 minutes. Supernatants were collected and CysLT levels quantified and measured in pg/mL. Data are presented as the mean ± SEM of a minimum of six separate studies. ‡p = 0.05, **p < 0.03, ***p < 0.002 as compared with unstimulated cells. Reprinted with permission of the Journal of Immunology. Source: Reference 66.