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. 2014 Nov 3;14(1):136–147. doi: 10.1074/mcp.M114.043513

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Fig. 1. — Top binding subsequences and peptides for eight tested monoclonal antibodies. The bar plots show the top binding subsequences (top panel) and subsequences (bottom panel) for each of the eight tested monoclonal antibodies. P53Ab1 (RHSVV), HA (DVPD), 4C1 (FDSH), and FLAG (DYDDDK) each had on-target motifs that were identified within each of the top binding peptides, and these were enhanced through subsequence analysis (shown in red). DM1A (ALEKD) had few on-target motifs in its top peptides, but subsequence analysis revealed the true epitope. FLAG cross-reacted most strongly with the epitope from DM1A (ALEKDY), but subsequence analysis successfully removed this effect.