Table I. Monoclonal antibodies used in this study.
| Epitope | Ab name | Immunogen | Isotype | pI | GRAVY | Mean signal intensity | Mapped predicatively |
|---|---|---|---|---|---|---|---|
| EEDFRV | A10 | Human Pol II | IgG2b | 4.1 | −1.3 | 4911 | No |
| SDLWKL | p53ab8 | Human p53 | IgG2b, IgG2a | 5.6 | −0.3 | 6243 | No |
| QAFDSH | 4C1 | Human insulin receptor | IgG2a | 5.1 | −1.1 | 971 | Yes |
| RHSVV | p53ab1 | Human p53 | IgG1 | 9.8 | 0 | 5074 | Yes |
| DYKDDDDK | FLAG | FLAG peptide | IgG1 | 4 | −3.3 | 1167 | Yes |
| AALEKD | DM1A | Human tubulin α | IgG1κ | 4.7 | −0.6 | 5798 | Yes |
| YPYDVPDYA | HA | HA peptide | IgG1 | 3.6 | −0.9 | 905 | Yes |
| NAHYYVFFEEQE | 2C11 | Human insulin receptor | IgG1 | 4.5 | −1 | 827 | No |
Monoclonal antibodies were used to test the motif search analysis algorithm. The highest rated subsequences were related to the true epitope and to each other to an extent that ensured the emergence of a conserved motif with strong association to the epitope sequence. Ab, antibody; GRAVY, grand average of hydropathicity index (25).