Table II. On-target versus off-target binding.
Total binders | On target | Fraction | |
---|---|---|---|
AB1 | 42,386 | 466 | 1.10 × 10−2 |
HA | 1608 | 53 | 3.30 × 10−2 |
4C1 | 2561 | 276 | 1.08 × 10−1 |
FLAG | 7563 | 0 | 0 |
DM1A | 44,821 | 207 | 4.62 × 10−3 |
A10 | 44,924 | 37 | 8.24 × 10−4 |
AB8 | 46,327 | 1 | 2.16 × 10−5 |
2C11 | 671 | 0 | 0 |
This table shows the number of peptides for each antibody that yielded a signal greater than 5-fold above background (“total binders”) and how many of those had at least 80% sequence identity with the true epitope (“on target”). See Table I for a list of true epitopes. A very low percentage (<11%) of the binding peptides had strong sequence similarity with the true epitope, in agreement with previous studies (24).