Fig. 2.

Glycan heterogeneity observed on Phl p 1 and Phl p 5 grass pollen allergens. A, Deconvoluted MS1 spectra of co-eluting glycoforms on the tryptic V²⁷PPGPNITATYGDK⁴⁰ (including one Hyp) and semitryptic I²⁴PKVPPGPNITATYGDK⁵⁰ (including two Hyp) peptides originating from Phl p 1. Glycoforms at and above m/z 2808.33 are found on the semitryptic peptide. B, Glycan heterogeneity of the Phl p 5 peptide containing six Hyp residues (indicated as p) and up to seven Pen glycans. All m/z values are represented as [M+H]⁺. The cartoons shown are based upon mass measurements and assumptions of the known biosynthetic pathways.