Table I. Glycopeptides from tryptic digest of natural grass pollen and dust mite allergens identified by bottom up mass spectrometry.
Allergen | Peptide | Modificationsa |
---|---|---|
Phl p 1 | V27PPGPNITATYGDK40 | Hyp1; Pen1dHex1Hex2–3HexNAc2 |
I24PKVPPGPNITATYGDK40 | Hyp2; Pen1–4dHex1Hex 2–3HexNAc2 | |
Phl p 5a | A26DLGYGPATPAAPAAGYTPATPAAPAEAAPAGK58 | Hyp6–7; Pen0,2 |
Phl p 5.0103 | A26DLGYGPATPAAPAAGYTPATPAAPAGAEPAGK58 | Hyp0–7; Pen0–7 |
Phl p 5b | A20DAGYAPATPAAAGAAAGK38 | Hyp2; Pen0–1 |
Der p 1 | N150QSLDLAEQELVDCASQHGCHGDTIPR176 | HexNAc1,2 |
E227ALAQTHSAIAVIIGIKDLDAFR249 | Hex | |
Der f 1 | N151TSLDLSEQELVDCASQHGCHGDTIPR177 | HexNAc1,2 |
R204PNSQHYGISNYCQIYPPDVKQIR227 | Hex | |
E228ALTQTHTAIAVIIGIKDLR247 | Hex | |
Der p 2 | G49KPFQLEAVFEANQNTKTAK68 | Hex1–4 |
C95PLVKGQQYDIK106 | Hex | |
G100QQYDIKYTWNVPK113 | Hex | |
I114APKSENVVVTVK126 | Hex | |
Der f 2 | G49KPFTLEALFDANQNTK65 | Hex1–9 |
C95PLVKGQQYDIK106 | Hex | |
G100QQYDIKYTWNVPK113 | Hex1,2 | |
I114APKSENVVVTVK126 | Hex |
a Variations in number of monosaccharide units shown in bold. Characters: - represent “between” and, represent “or”. Hyp, hydroxyproline; Pen, pentose; dHex, deoxyhexose; Hex, hexose; HexNAc, N-acetylhexosamine.