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. 2014 Oct 17;14(1):50–65. doi: 10.1074/mcp.M114.039370

Fig. 1.

Fig. 1.

NGF treatment leads to JNK-dependent neurite formation in PC12 cells. A, treatment with NGF (100 ng/ml) induces a major shift in the phenotype of PC12 cells from proliferating tumor cells to non-dividing neurons showing growth of neurites at 6 h after stimulation. Pretreatment with the JNK inhibitor SP600125 (10 μm) for 1 h led to a significant decrease in neurite outgrowth. **p < 0.01, ***p < 0.001 versus unstimulated or NGF treated (n = 3 each). Scale bar: 20 μm. B, neurite outgrowth was accompanied by NGF-induced JNK phosphorylation (∼3-fold increase) peaking 2 h after NGF treatment. NGF treatment had no effect on total JNK levels. Arrows indicate the two different p54 and p46 JNK isoforms. **p < 0.01 versus unstimulated (n = 4 to 7).