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. 2014 Oct 17;14(1):50–65. doi: 10.1074/mcp.M114.039370

Fig. 4.

Fig. 4.

JNK1 interacts with the Nono–Sfpq heterodimer in a d-JNKI-1- and RNA-dependent way in NGF-treated PC12 cells. A, JNK1 was immunoprecipitated from whole cell lysates in unstimulated or NGF-treated cells and in the presence or absence of d-TAT or d-JNKI-1. Western blots against Nono, Sfpq, and Pspc1 showed that d-JNKI-1 decreased NGF-induced interaction of JNK1 with these proteins. A representative blot is shown. B, Nono was immunoprecipitated from whole cell lysates of unstimulated or NGF-treated cells and in the presence or absence of RNase A. Densitometric analysis of Western blots showed a ∼2.5-fold increase in JNK1–Nono interaction after 3 h of NGF treatment, which was inhibited by RNase A treatment (*p < 0.05 versus unstimulated (n = 4)). Interaction of Sfpq and Nono was not affected by this treatment. Reprobing with JNK2-specific antibody did not show any detectable signal in the more prominent JNK2 band p54. PC12 whole cell lysate (WCL) was used as a positive control.