Skip to main content
. 2014 Oct 17;14(1):50–65. doi: 10.1074/mcp.M114.039370

Fig. 5.

Fig. 5.

Co-localization and cellular distribution of Nono, Sfpq, and JNK1. A, confocal microscopy images of Nono (green), Sfpq (red), and nuclei (blue) co-stained PC12 cells show that Nono and Sfpq were expressed in the nucleus as well as in the cytosol. Co-localization mainly occurred in the nucleus, where the greater fraction of Nono and Sfpq is located (upper panel). The cellular distribution of JNK1 (green) showed that JNK1 was mainly localized in the cytosol (lower panel). NGF treatment for 3 h led to an increased co-localization signal of JNK1 (green) and Sfpq (red) in the cytosol. Scale bar: 6 μm. B, Western blot analysis of cytosolic and nuclear fractions revealed that NGF treatment for 3 h led to JNK phosphorylation mainly in the cytosol. Reprobing with JNK1- and JNK2-specific antibodies showed that JNKs were mainly localized in the cytosol, whereas the JNK2 p54 isoform was also found in the nucleus. Reprobing with Nono- and Sfpq-specific antibodies corroborated the cellular distribution of Nono and Sfpq noted in confocal microscopy analysis. Representative immunoblots are shown (n = 2).