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. 2014 Oct 17;14(1):50–65. doi: 10.1074/mcp.M114.039370

Fig. 6.

Fig. 6.

Co-localization and cellular distribution of Nono, Sfpq, and JNK1 assessed via proximity ligation assay (PLA). PC12 cells were incubated with Nono- and Sfpq-specific antibodies followed by PLA secondary antibodies (red) and were analyzed via confocal microscopy. Nuclear counterstaining with DAPI (blue) revealed that the Nono–Sfpq heterodimer was mainly located in the nucleus (upper panel). Incubation with JNK1- and Sfpq-specific antibodies followed by PLA staining showed a ∼5.4-fold increase in JNK1–Sfpq interaction after NGF treatment for 3 h (lower panel). **p < 0.01 versus unstimulated (n = 8 to 9). Nuclear counterstaining with DAPI showed that JNK1 and Sfpq mainly interacted in the cytosol of NGF-treated cells. Results were obtained from three independent experiments. Scale bar: 6 μm.