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. 2014 Oct 17;14(1):50–65. doi: 10.1074/mcp.M114.039370

Fig. 8.

Fig. 8.

Knockdown of Sfpq led to decreased NGF-induced neurite outgrowth. A, PC12 cells were transfected with different amounts of Sfpq siRNA. Quantification of band intensities of PSF Western blots showed a knockdown efficiency after 4 days of 97% using 200 nmol of Sfpq siRNA. B, PC12 cells were mock-transfected or transfected with 200 nmol Sfpq siRNA for 4 days. NGF-induced neurite outgrowth in PSF knockdown cells was decreased by ∼50%, whereas 10 μm SP600125 inhibited NGF-induced neurite formation in mock-transfected cells by ∼69%. Pretreatment with SP600125 of Sfpq knockdown cells did not significantly further decrease NGF-induced neurite outgrowth. *p < 0.05, **p < 0.01 versus NGF treated; n.s. = non-significant (n = 3 each).