Fig. 5.

Proteomic analysis of Skp1 interactions. Slug-stage cells were subjected to co-IP with affinity-purified anti-Skp1 (UOK77)-coupled beads. Data from four co-IPs conducted under two conditions (pH 7.4 or pH 8.0; 0.2% or 1% Nonidet P-40), each conducted in triplicate, were pooled owing to the absence of systematic differences, and total spectral counts are reported. A, Ax3 and phyA^– are compared. Asterisks denote those proteins for which the quantitative difference between wt and phyA^– cells was observed in all trials in which the protein was detected. B, comparison of phyA^–, gnt1^–, pgtA^–, and agtA^– and wt (Ax3) cells. Protein descriptors and RNA expression patterns are in supplemental Table S1. Full data are shown in supplemental Table S3.