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Epitope-tagged Salmonella effector protein SseF is an integral component of SMMs, indicated by its co-localization with LAMP1. HeLa cells constitutively expressing LAMP1-GFP (green) were infected with Salmonella HH107 expressing SseF-2TEV-2M45 fixed at 8 h p.i., immunostained against Salmonella O antiserum (red) and the M45 tag (blue), and imaged using CLSM. Images are shown as maximum-intensity projections. Scale bar: 10 μm.
