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. 2015 Jan 9;5:441. doi: 10.3389/fgene.2014.00441

FIGURE 2.

FIGURE 2

Anticancer effects of RECQL1-siRNA and WRN-siRNA. (A) Time course experiments measuring the inhibitory effect of the RECQL1-siRNA-LIC101 complex on the growth of Hep3B cancer nodules. Open squares, red triangles and closed circles show mice treated with 10% maltose solution as a control, NS(non-silencing)-siRNA/LIC101 complex and RECQL1-siRNA/LIC101 complex, respectively. Arrow heads represent injections made subcutaneously into cancer-bearing mice. The anticancer effect of RECQL1-siRNA/LIC101 on Hep3B cancer-bearing mice is represented visually. Panels (A–C) represent the size of cancer nodules grown until 42 days after inoculation. Reprinted from the paper by Futami et al. (2008b). (B) Anticancer effects of locally injected WRN-siRNA and RECQL1-siRNA on head and neck squamous cell carcinoma, and the synergistic effect made by the simultaneously intravenously injected genotoxic platinum drug CDDP. Therapeutic effects of WRN-siRNA and RECQL1-siRNA, and the combined effect with CDDP were examined in human FaDu carcinoma-bearing mouse xenografts. NC, no siRNA addition; GL3, GL3-siRNA; RECQL1, RECQL1-siRNA; WRN,WRN-siRNA. The right panel shows immunohistochemical evaluations of WRN and RECQL1 proteins expressed in surgically isolated cancerous regions of a human FaDu carcinoma-bearing xenograft mouse model after treatment with injections of the siRNA-atelocollagen complex. GL3-siRNA represents control siRNA that does not inhibit the expression of WRN or RECQL1 helicases. WRN-siRNA and RECQL1-siRNA treatments reduced the expression of WRN and RECQL1 proteins, respectively, stained by specific antibodies. Reprinted from the paper by Arai et al. (2011) by courtesy of those authors. (C) Increased cytotoxicity of anticancer drug camptothecin (CPT) in cancer cells by co-treatment with WRN-siRNA. HeLa cells were transfected with a sub-lethal concentration of WRN-siRNA and were treated with a serial dilutions of CPT added after transfection. After 48 h of culture, the cell viability was monitored. Reprinted from the paper by Futami et al. (2007). The half-lethal dose (LD 50%) of CPT was reduced by nearly ten-fold by treatment with WRN-siRNA (10 μM). Reprinted from the paper by Futami et al. (2007).