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Flow cytometry analysis of isolated ADSCs after rapid isolation (no collagenase). Cells stained (A) 85.8% positive for CD90, (B) 99.9% positive for CD73, (C) 99.4% positive for CD105 and (D) 3.79% positive for CD14, CD20, CD34 and CD45. (E) Collagenase-isolated ADSCs after 6 days of primary culture seeded at 5 × 105 in T75 flask. (F) Rapid isolation ADSCs after 6 days primary culture seeded at 2 × 106 in T75 flask.
