Fig. 3.

Effect of DHGA-D on UVB-induced phosphorylation of MAPKs in HaCaT cells. (A) and (B) DHGA-D inhibits UVB-induced phosphorylation (p-) of MKK3/6, p38, Elk-1, MKK4, JNK1/2, c-Jun, and MSK1 in HaCaT cells. Cells were pre-treated with DHGA-D at the indicated concentrations (2.5 and 5 μM) for 1 hr, stimulated with UVB irradiation, and harvested 15 or 30 min. later. Phosphorylation was detected by Western blotting assay with specific antibodies. Data are representative of three independent experiments that gave similar results.