Fig. 5.

Effect of MLK3 knockdown on UVB-induced COX-2 expression, as well as MKK3/6 and MKK4 phosphorylation in HaCaT cells. (A) Knockdown of MLK3 reduces UVB-induced COX-2 expression in HaCaT cells. Expression levels of COX-2 were determined by Western blot assay with specific antibodies. (B) Knockdown of MLK3 reduces UVB-induced MKK3/6/JNK1/2 and MKK4/p38 phosphorylation in HaCaT cells. Phosphorylation was detected by Western blot assay with phosphor-specific antibodies. Data are representative of three independent experiments that gave similar results.