Fig. 1.

Compounds used in this article and their HDACi activity. (A) Chemical structures of chiral hydroxamic-based compounds (S)-8 and (R)-8. (B) HDACi activity of the two enantiomers was comparatively assessed in A375 melanoma cells which were first seeded in 6-well plates (10⁵ cell/well) and allowed to attach overnight. On the next day cultures were added without/with 5 μM (S)-8 or (R)-8 and maintained for 6, 15 and 24 hrs when cells were detached and extracted by sonication. Cell extracts were normalized for protein content and then processed by Western blot; immunostaining of acetylated forms of histones H3 and H4 as well as of α-tubulin and p53 were revealed with specific antibodies; GAPDH was used as the loading control.