Fig. 5.

(S)-8 decreases motility, invasiveness, migration and angiogenic potential of A375 cells in vitro. (A) (S)-8 inhibited A375 cell motility. Confluent cultures were ‘wounded’ with the aid of a sterile plastic tip and maintained without/with increasing amounts of drug for 24 hrs. A phase-contrast microscopy was used to take pictures of the monolayers (magnification ×100). (B, left) Aliquots of conditioned media from A375 cultures incubated without/with increasing amounts of (S)-8 for 24 hrs in the absence of FCS were submitted to gelatin zymography and then to densitometric analysis to quantify MMP-2 activity that was reported as% of control. (B, right, C and D) MMP-2, TIMP-1, TIMP-2, VEGF-A and VEGF-R2 mRNA levels, from A375 cells treated without/with 2.5–5 μM (S)-8 for 24 hrs were assessed by quantitative real-time PCR (***P ≤ 0.001).